Fig. 3
From: Effective production of kojic acid in engineered Aspergillus niger
Construction of A. niger strains with RNA interference targeting ASPNIDRAFT_42619 and ASPNIDRAFT_56871 respectively. a Illustration of RNAi cassettes designed with inverted repeats of 500Â bp of coding sequence of the gene of interest separated by a spacer segment of GFP sequence. pLH1738 was used to interfere with ASPNIDRAFT_42619 expression, pLH1739 to interfere with ASPNIDRAFT_56871 expression. b qRT-PCR analysis of target gene expression for the parent strain S2132 and the RNAi strains. the expression of ASPNIDRAFT_42619 and ASPNIDRAFT_56871 was interfered with in S2930 and S2933 respectively. Results were first standardized against actin, with S2132 expression set arbitrarily to 1