Table 2 Bacterial strains and plasmids used in this study

XL1-Blue

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIqZ∆M15 Tn10 (Tet^r)]

Stratagene^a

C. glutamicum

Wild type

ATCC 13032

Plasmids

Relevant Characteristics

Ref. or Source

pCES208

E. coli-C. glutamicum shuttle vector; Km^R

[43]

pCG-H36A

pCES208 derivative; P_H36, Signal sequence of cg1514

[14]

pCES-H36-GFP

pCES208 derivative; P_H36, GFP

[12]

pCES208-SapI

pCES208 derivative; two additional SapI restriction enzyme sites

This study

pA

pCES208 derivative; P_H36, phaA from R. eutropha, rrnB T1T2 terminator

This study

pHA

pCES208 derivative; P_H36, phaA from R. eutropha with N-terminal 6 × His tag, rrnB T1T2

This study

pUHA

pCES208 derivative; P_H36, T7 g10 RBS, phaA from R. eutropha with N-terminal 6 × His tag, rrnB T1T2

This study

pB

pCES208 derivative; P_H36, phaB from R. eutropha, rrnB T1T2 terminator

This study

pHB

pCES208 derivative; P_H36, phaB from R. eutropha with N-terminal 6 × His tag, rrnB T1T2 terminator

This study

pUHB

pCES208 derivative; P_H36, T7 g10 RBS, phaB from R. eutropha with N-terminal 6 × His tag, rrnB T1T2

This study

pC

pCES208 derivative; P_H36, phaC from R. eutropha, rrnB T1T2 terminator

This study

pHC

pCES208 derivative; P_H36, phaC from R. eutropha with N-terminal 6 × His tag, rrnB T1T2 terminator

This study

pUHC

pCES208 derivative; P_H36, T7 g10 RBS, phaC from R. eutropha with N-terminal 6 × His tag, rrnB T1T2

This study

pABC

pCES208 derivative; P_H36-T7 g10 RBS-phaA with N-terminal 6 × His tag-rrnB T1T2; P_H36-T7 g10 RBS-phaB with N-terminal 6 × His tag-rrnB T1T2; P_H36-T7 g10 RBS-phaC with N-terminal 6 × His tag-rrnB T1T2

This study

pABC-S

pABC derivative; P_H36-BCD2-fbp-rrnBT1T2; P_H36-BCD2-acnR-rrnBT1T2; P_H36-BCD2-mez-rrnBT1T2

This study

Glu#6

pCES208 derivative; P_I64-BCD21-fbp-rrnBT1T2; P_I64-BCD21-acnR-rrnBT1T2; P_H36-BCD8-mez-rnBT1T2

This study

Fru#1

pCES208 derivative; P_H17-BCD8-fbp-rrnBT1T2; P_L10-BCD21-acnR-rrnBT1T2; P_H30-BCD8-mez-rrnBT1T2

This study