Fig. 6
Engineering carbon substrate-specific metabolic networks in C. glutamicum for PHB production. A FACS-based screening of the metabolic network library using fructose as a carbon substrate. The histograms of negative control (pCES208), original library, 1st sorted library, 2nd sorted library, and 3rd sorted library cells are represented by filled-gray, tinted-blue, orange, red, and purple lines, respectively. B PHB production levels using glucose or fructose as a sole carbon source in C. glutamicum strains harboring empty vector (pCES208), engineered PHB synthesis pathways (pABC and pABC-S), and the isolated clones from the metabolic network library (Glu#6 and Fru#1). All measurements are based on two biological replicates. Error bars represent standard deviation of the biological replicates