Fig. 5

FACS-based high-throughput screening of metabolic network library for enhanced production of PHB A Schematic diagram of FACS-based screening strategy of the metabolic network library for enhanced production of PHB. B FACS-based screening of metabolic network library using glucose as a carbon substrate. The histograms of negative control (pCES208), original library, 1st sorted library, and 2nd sorted library cells are represented by filled-gray, tinted-blue, orange, and red lines, respectively. C PHB production levels using glucose as a sole carbon source in C. glutamicum strains harboring empty vector (pCES208), engineered PHB synthesis pathways (pABC and pABC-S), and the best clone isolated from metabolic network library (Glu#6). All measurements are based on two biological replicates. Error bars represent standard deviation of the biological replicates