Fig. 4

Metabolic network of PHB biosynthesis in C. glutamicum and gene expression in bicistronic design. A Metabolic network optimization for PHB synthesis by fine-tuning of gene expression levels of the fbp, acnR, mez genes. B Schematic illustration of bicistronic expression system with 20 synthetic promoters and bicistronic design of RBS (BCD) in C. glutamicum. C Comparison of GFP gene expression using six synthetic promoters and three BCDs in bicistronic expression system