Fig. 3

Fluorescence-based quantification assay for PHB content in C. glutamicum. A Intracellular PHB contents are linearly correlated with fluorescence intensities of BODIPY-stained PHB granules in cells. B Confocal microscope images of BODIPY-stained PHB-producing cells. (i) C. glutamicum harboring pCES208, 15 h cultivation in minimal media containing 2% glucose (PHB content: 0 wt%); (ii) C. glutamicum harboring pABC, 15 h cultivation in minimal media containing 2% glucose (PHB content: 3 wt%); (iii) C. glutamicum harboring pABC, 15 h cultivation in BHI media containing 2% glucose (PHB content: 12 wt%); iv, C. glutamicum harboring pABC, 36 h cultivation in BHI media containing 2% glucose (PHB content: 25 wt%)