Fig. 1
Engineering R. eutropha PHB synthesis pathway in C. glutamicum. A PHB biosynthetic metabolic pathway. The thick red arrows represent overexpression of genes in the PHB synthesis pathway. Glc glucose, Pyr pyruvate, AcCoA acetyl-CoA, AcAcCoA acetoacetyl-CoA, R-3-HBCoA R-3-hydroxybutyrl-CoA, PHB polyhydroxybutyrate, TCA tricarboxylic acid cycle. B Expression systems for phaA, phaB, phaC genes (left panel) and SDS-PAGE analysis of gene expression in C. glutamicum (right panel). PH36 H36 synthetic promoter, rrnBT rrnBT1T2 terminator, H N-terminal 6 × His tag, T7RBST7 gene10 RBS. Lane N, negative control C. glutamicum cells harboring pCES208 empty vector; Lanes 1 to 3, Expression systems 1 to 3; Lane M, Molecular weight markers. White, black and gray triangles indicate the bands of PhaC, PhaA and PhaB, respectively