Table 4 Hydrogen evolution efficiency (mLH2/g RS), hydrogen yield (mL/g DW) and hydrogen rate (mL/L/h) from K. pneumoniae and E. cloacae and their mixed coculture using hydrolysates O. acuminata of by different treatments
Cultures | K. pneumoniae | E. cloacae | coculture | |||||
|---|---|---|---|---|---|---|---|---|
Properties | Glucose (15 g/L) | Thermochemical pretreated O. acuminata (35 g/L) | Glucose (15 g/L) | Thermochemical pretreated O. acuminata (35 g/L) | Glucose (15 g/L) | Thermochemical pretreated O. acuminata (35 g/L) | Biological pretreated O. acuminata (35 g/L) | Biological treated + NPs O. acuminata (35 g/L) |
Initial sugar (g/L) | 15 ± 0.47 | 16 ± 0.57 | 15 ± 0.47 | 16 ± 0.57 | 15 ± 0.47 | 16 ± 0.57 | 31 ± 0.54 | 45 ± 1.36 |
Residual sugar (g/L) | 3.1 ± 0.17 | 5.2 ± 0.27 | 3.2 ± 0.12 | 5.2 ± 0.65 | 2.6 ± 0.12 | 4.6 ± 0.21 | 17 ± 0.63 | 29.34 ± 1.07 |
Consumed sugar (g/L) | 11.9 ± 0.13 | 10.8 ± 0.63 | 11.8 ± 0.39 | 10.8 ± 0.64 | 12.4 ± 0.8 | 11.4 ± 0.76 | 14 ± 0.52 | 15.66 ± 0.9 |
Total hydrogen (mL H2/g RS) | 87.9 ± 2.3 | 94.6 ± 3.28 | 70.8 ± 1.6 | 75.3 ± 2.5 | 104.8 ± 3.6 | 133.3 ± 4.2 | 188.9 ± 5.3 | 216.9 ± 4.69 |
Hydrogen yield (mL H2/g DW) | 69.8 ± 1.2 | 29.2 ± 0.8 | 55.7 ± 2.3 | 23.2 ± 1.7 | 86.7 ± 2.2 | 43.4 ± 1.6 | 75.6 ± 1.6 | 92.9 ± 1.68 |
hydrogen rate (mLH2/L/h) | 14.52 ± 0.6 | 14.19 ± 0.91 | 11.5 ± 0.62 | 11.2 ± 0.25 | 27.08 ± 1.03 | 31.66 ± 2.01 | 55.10 ± 2.1 | 67.7 ± 3.4 |