Application of a novel biological-nanoparticle pretreatment to Oscillatoria acuminata biomass and coculture dark fermentation for improving hydrogen production

Table 3 Biohydrogen production (mLH₂/L) from coculture of K. pneumoniae and E. cloacae using hydrolysate of O. acuminata by biological treatment

Incubation period (h)	Glucose	Biological treatment	Biological treatment + Mg-Zn Fe₂O₄ NPs
12	500 ± 20.45	500 ± 22.65	590 ± 36.45
24	960 ± 30.25	1765 ± 23.25	2214 ± 70.21
36	1160 ± 32.89	2134 ± 37.25	2954 ± 66.3
48	1300 ± 20.56	2645 ± 31.02	3254 ± 36.54
F-value	2653.2*	1632.6*	9561*

Values represent means of three replicates ± standard error; * indicate significant differences (p ≤ 0.05)

ISSN: 1475-2859