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Table 1 Measurement of lipid content, titre, and biomass productivity

From: Mastering targeted genome engineering of GC-rich oleaginous yeast for tailored plant oil alternatives for the food and chemical sector

  

Lipid

Biomass productivity g/Lh

  

Content % w/w

SD

Titre g/l

SD

Productivity g/Lh

SD

After 96 h

SD

After 72 h

SD

MNM + Glu

WT

67.7

2.84

8.26

0.85

0.11

0.01

0.13

0.01

0.12

0.01

D9OE

67.5

3.93

8.48

1.79

0.12

0.02

0.15

0.00

0.13

0.00

TEFp-D9

57.2**

1.36

4.64**

0.62

0.06*

0.01

0.08**

0.01

0.08*

0.01

AKRp-D9

54.6*

3.26

4.20**

0.12

0.06*

0.00

0.08**

0.00

0.07*

0.00

ΔD12

61.3

2.20

4.03**

0.65

0.06*

0.01

0.07**

0.01

0.07**

0.01

RM + AA + Glu

WT

80.1

1.82

25.1

1.96

0.35

0.03

0.47

0.07

0.43

0.03

D9OE

79.6

8.11

38.8**

0.09

0.54**

0.00

0.79*

0.06

0.68*

0.07

TEFp-D9

68.6*

3.15

24.9

2.04

0.35

0.03

0.58

0.07

0.50

0.05

AKRp-D9

50.2***

2.04

13.0**

0.14

0.18**

0.00

0.46

0.02

0.36*

0.01

ΔD12

89.6

4.66

33.3*

1.49

0.41

0.08

0.64*

0.00

0.52

0.01

  1. Lipid analysis was performed after reaching the stationary phase in each medium. In the RM + AA + Glu medium, the analysis was performed after 72 h of cultivation, and in the MNM + Glu medium after 96 h of cultivation in the fed-batch fermentations. The biomass productivity was determined in both media after 72 h and 96 h of cultivation. All data represent averages of biological replicates, and significant differences between the WT strain and each engineered C. oleaginosus strain were defined using the two-tailed Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
  2. SD standard deviation
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Microbial Cell Factories

ISSN: 1475-2859

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