Fig. 4

Survival of P. putida DOT-T1E mutants grown on glucose (A and C) or grown on glucose with styrene in the gas phase (B and D) after 60 min exposure to trans-cinnamic acid (50 mM) or styrene (0.1% (v/v)). Panel A and Panel B show mutants highly sensitive to a sudden styrene shock when grown on glucose, while C and D show mutants as sensitive as the wild-type strain to styrene when grown on glucose. Control without addition, orange bars; plus 50 mM trans-cinnamic acid, yellow bars; plus 0.1% (v/v) styrene, green bars