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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Structure-function analysis of CYP719As involved in methylenedioxy bridge-formation in the biosynthesis of benzylisoquinoline alkaloids and its de novo production

Fig. 5

Conversion rates of CyCYP719As mutants relative to wild-type CyCYP719As. A Conversion rates of CyCYP719As mutants in catalyzing the D ring formation using (S)-scoulerine as substrate to produce (S)-cheilanthifoline. B Conversion rates of CyCYP719As mutants in catalyzing the A ring formation using (S)-scoulerine as substrate to produce (S)-nandinine. C Conversion rates of CyCYP719As mutants in catalyzing the A ring formation using (S)-cheilanthifoline as substrate to produce (S)-stylopine. D Conversion rates of CyCYP719As mutants in catalyzing the D ring formation using (S)-nandinine as substrate to produce (S)-stylopine. Data reported are the means ± SD from triplicate analyses. ** indicates P < 0.01; nd, not detected. The experimental data are shown in Additional file 1: Table S4

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Microbial Cell Factories

ISSN: 1475-2859

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