Fig. 1

Synthetic pathway of cembratriene-ol in E. coli. F6P, fructose 6-phosphate; G3P, glyceraldehyde 3-phosphate; PYR, pyruvate; DXP, 1-deoxy-d-xylulose-5-phosphate; MEP, 2-C-methyl-d-erythritol-4-phosphate; CDP-ME, 4-(cytidine 5’-diphospho)-2-C-methyl-d-erythritol; CDP-MEP, 2-phospho-4-(cythidine 5’-diphospho)-2-C-methyl-d-erythritol; MEcPP, 2-C-methyl-d-erythritol 2,4-cyclodiphosphate; HMBPP, 1-hydroxy-2-methyl-2-butenyl 4-diphosphate; IPP, 4-hydroxyphenylpyruvate; DMAPP, dimethylallyl diphosphate; GPP, geranyl diphosphate; FPP, farnesyl diphosphate; GGPP, geranylgeranyl diphosphate; dxs, 1-deoxy-d-xylulose 5-phosphate synthase gene; dxr, 1-deoxy-d-xylulose 5-phosphate reductoisomerase gene; IspD, 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase gene; IspE, 4-(cytidine 5’-diphospho)-2-C-methyl-d-erythritol kinase gene; IspF, 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase gene; IspG, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase gene; IspH, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase; idi, isopentenyl-diphosphate Δ-isomerase gene; ispA, geranyl diphosphate synthase gene