Metabolic stress constrains microbial L-cysteine production in Escherichia coli by accelerating transposition through mobile genetic elements

Table 3 Plasmids used in this study: All plasmids are derived from pACYC184 with a p15A origin of replication and approx. 15 copies/cell

Plasmid	Relevant features	References
pCYS	p_GAPDH:ydeD, p_serAp1,2:serA317, p_cysE: cysE-XIV, tet^R, p15A	[26, 27, 29, 30]
pCYS_i	p_GAPDH:cysE-XIV-ydeD-serA317, tet^R p15A	This study
pCYS_m	p_GAPDH:ydeD, p_serAp1,2:serA317, p_fic:cysM, p_cysE: cysE-XIV, tet^R, p15A	This study

ISSN: 1475-2859