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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing method

Fig. 5

Overview of the RAPID genome editing system. a Principle of the currently available one-step CRISPR technologies. b Principle of the RAPID technology, wherein CRISPR-crRNA is used as a counterselection, which significantly increases the number of edited colonies owing to the high-efficiency HR mediated by RecET. c Workflow of the RAPID technology, wherein the transformation of linear donor fragments and pXM-sacB-crRNA can be used for gene deletion. The whole process is simplified and comparable to that of the one-step CRISPR genome editing system

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Microbial Cell Factories

ISSN: 1475-2859

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