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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing method

Fig. 3

RecET-mediated HR and IPTG-inducible Cpf1 genomic cleavage, potentially applicable for the development of a two-step CRISPR–Cpf1 system. a Linear templates with different lengths are used for integration, and kanamycin-resistant colonies represent the HR activity. b A decrease in the number of colonies after IPTG addition represents a strong inducible effect. The colonies were counted or calculated based on dilution. Experiments were performed in triplicates. Values are presented as mean ± SD. **P < 0.01, *** P < 0.001; n.s., not significant

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Microbial Cell Factories

ISSN: 1475-2859

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