Fig. 1From: Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing methodEffects of the expression of different recombinases on HR of DNA fragment into the genome. A 528 bp DNA fragment flanked by approximately 500 bp genomic-homologous upstream and downstream arms was used. The integration efficiency was reflected by the colonies formed on kanamycin agar plates, which were counted or calculated based on dilution. Experiments were performed in triplicates. Values are presented as mean ± SD. ***P < 0.005Back to article page