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Table 2 The yield of each Crp isoform

From: Efficient recombinant production of mouse-derived cryptdin family peptides by a novel facilitation strategy for inclusion body formation

Purification step

Yield (mg/L of culture)

Crp1

Crp2

Crp3

Crp4

Crp6

CIEX

Crude extract

5.0

5.2

5.3

8.5

9.1

RP-HPLC

Crps

1.5

0.95

0.92

1.9a

1.8

Formyl-Crps

1.7

2.3

2.3

5.3

3.0

Deformylation

Crps

1.4

2.0

1.9

4.2

2.2

Total Crps

2.9

3.0

2.8

6.1a

4.0

  1. The total yield of Crps was obtained by adding the amount of Crps after purification by RP-HPLC to the amount of Crps after deformylation
  2. aCrp4 represents the sum of the total amounts of Crp4 with and without methionine
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Microbial Cell Factories

ISSN: 1475-2859

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