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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Efficient recombinant production of mouse-derived cryptdin family peptides by a novel facilitation strategy for inclusion body formation

Fig. 5

Co-expression of Crps using the E. coli Origami™ B (DE3) strain. a Schematic outline of the newly investigated method for promoting inclusion body formation by disulfide bond cross-linking using the E. coli Origami™ B (DE3) strain. HLA, green; Crp, red; disulfide cross-link, yellow. Use of the Origami™ B (DE3) strain, which has an intracellular oxidative environment, as an expression host allows the formation of non-natural disulfide cross-links between aggregation-prone HLA and Crp. This is expected to result in more efficient inclusion body formation. b Tricine-SDS-PAGE results of the expression of Crps. Lane M: marker; Lanes 1–6: precipitates of Crp1–6. Lane7: Precipitate of Crp4 by BL21 strain was used as a control. c The intensity data of the expression of Crps relative to Crp4 using the BL21(DE3) strain. n = 3 for each

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Microbial Cell Factories

ISSN: 1475-2859

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