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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Efficient recombinant production of mouse-derived cryptdin family peptides by a novel facilitation strategy for inclusion body formation

Fig. 3

Co-expression of Crps using the E. coli BL21(DE3) strain. a Schematic outline of the method for promoting inclusion body formation using the BL21(DE3) strain. HLA, green; Crp, red. When co-expressed with aggregation-prone HLA, Crp forms a stable inclusion body (black arrow). If it does not form, host proteases degraded the expressed Crp (gray arrows). b Tricine-SDS-PAGE results of the expression level of Crps. Lane M: marker; Lanes 1–6: precipitates of Crp1–6. c The intensity data of the expression of Crps relative to Crp4. n = 3 for each

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Microbial Cell Factories

ISSN: 1475-2859

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