Production and bioprocessing of camptothecin from Aspergillus terreus, an endophyte of Cestrum parqui, restoring their biosynthetic potency by Citrus limonum peel extracts

Table 2 Plackett–Burman experimental design for evaluating the effect of nineteen-independent variables on the actual and predicted camptothecin yield from Aspergillus terreus

Run	X1	X2	X3	X4	X5	X6	X7	X8	X9	X10	X11	X12	X13	X14	X15	X16	X17	X18	X19	Actual yield	Predicted yield	Residuals
1	10	2	20	2	2	0.1	2	10	20	10	20	4	4	4	10	10	8	20	2	50.02	48.50	− 1.486
2	5	5	10	2	2	0.1	4	10	15	20	20	2	4	8	10	10	8	10	5	170.09	168.33	1.727
3	5	5	20	2	5	0.5	2	5	20	20	20	4	4	8	4	10	4	10	2	20.24	20.50	− 0.266
4	5	2	20	5	2	0.5	4	5	15	20	20	4	10	4	10	5	8	10	2	20.03	20.50	− 0.476
5	5	5	20	5	5	0.1	4	5	20	10	10	2	4	8	10	5	8	20	2	80	110.36	− 3.363
6	10	5	10	5	2	0.5	2	5	15	10	20	4	4	8	10	5	4	20	5	30.11	20.50	0.604
7	10	5	20	2	5	0.1	4	5	15	10	10	4	10	4	10	10	4	10	5	10.52	20.50	− 0.986
8	10	5	10	2	5	0.5	4	10	15	20	10	4	4	4	4	5	8	20	2	40.03	20.50	1.524
9	5	2	20	5	5	0.5	2	10	15	20	10	2	4	4	10	10	4	20	5	70.48	110.36	− 3.883
10	5	5	10	5	2	0.1	2	5	20	20	10	4	10	4	4	10	8	20	5	30.56	20.50	1.054
11	10	5	10	5	5	0.1	2	10	20	20	20	2	10	4	10	5	4	10	2	120.6	110.36	1.237
12	10	5	20	5	2	0.5	2	10	15	10	10	2	10	8	4	10	8	10	2	130.1	110.36	1.827
13	5	2	10	2	5	0.5	2	10	20	10	10	4	10	8	10	5	8	10	5	20.12	20.50	− 0.386
14	5	5	20	2	2	0.5	4	10	20	10	20	2	10	4	4	5	4	20	5	70.05	110.36	− 4.313
15	10	2	20	5	2	0.1	4	10	20	20	10	4	4	8	4	5	4	10	5	30.8	20.50	1.294
16	10	2	10	2	2	0.5	4	5	20	20	10	2	10	8	10	10	4	20	2	60.14	110.36	− 5.223
17	5	2	10	2	2	0.1	2	5	15	10	10	2	4	4	4	5	4	10	2	160.01	110.36	4.647
18	5	2	10	5	5	0.1	4	10	15	10	20	4	10	8	4	10	4	20	2	10.63	20.50	− 0.876
19	10	2	20	2	5	0.1	2	5	15	20	20	2	10	8	4	5	8	20	5	110.23	110.36	− 0.133
20	10	2	10	5	5	0.5	4	5	20	10	20	2	4	4	4	10	8	10	5	140.84	110.96	3.477

The “−1′′ sign correspond to the minimum value and the “+1′′ sign correspond to the maximum value of the input parameter range

ISSN: 1475-2859