Table 4 Substrate specificity of purified T. harzianum S7113 laccase isoenzymes
Substrates | Concentration (mM) | Optimal pH | Molar extinction coefficient (ɛ) (M−1 cm−1) | Relative activity (%) | |
|---|---|---|---|---|---|
Lac A | Lac B | ||||
ABTS | 0.3 | 3.0 | 36,000 | 0.36 ± 0.01 | 0.12 ± 0.001 |
DMP | 5.0 | 5.5 | 49.6 | 0.13 ± 0.006 | 0.05 ± 0.007 |
SGZ | 5.0 | 6.0 | 65.00 | 0.24 ± 0.009 | 0.12 ± 0.009 |
Guaiaciol | 5.0 | 5.5 | 12.000 | 0.08 ± 0.013 | 0.02 ± 0.001 |
Catechol | 5.0 | 5.0 | 2.211 | 0.03 ± 0.001 | 0.03 ± 0.007 |
Pyrogallol | 5.0 | 7.0 | 4.40 | 0.04 ± 0.002 | 0.016 ± 0.00 |