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Table 3 Effect of Inhibitors on the activity of T. harzianum S7113 purified laccase isoenzymes

From: Purification and biochemical characterization of two laccase isoenzymes isolated from Trichoderma harzianum S7113 and its application for bisphenol A degradation

Inhibitors

Concentration (mM)

Relative activity (%)

Lac A

Lac B

NaN3

0.1

0.23 ± 0.02

0.69 ± 0.73

0.5

0.00 ± 0.00

0.00 ± 0.00

EDTA

0.1

84.4 ± 2.62

97.7 ± 2.06

0.5

56.6 ± 1.87

93.6 ± 1.66

SDS

0.1

55.5 ± 1.93

94.7 ± 1.65

0.5

50.9 ± 2.45

63.9 ± 4.35

β-mercaptoethanol

0.1

52.1 ± 4.37

63.4 ± 3.01

0.5

10.0 ± 2.47

7.90 ± 2.39

  1. The enzyme was pre-incubated with different inhibitors for 10 min to ascertain the impact of inhibitors on laccase activity. Following the addition of the reaction's substrate (ABTS), the residual activity was assessed
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Microbial Cell Factories

ISSN: 1475-2859

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