Microbial Cell Factories

Table 2 Effect of metal ions on activity of T. harzianum S7113 purified laccase isoenzymes

From: Purification and biochemical characterization of two laccase isoenzymes isolated from Trichoderma harzianum S7113 and its application for bisphenol A degradation

Metal ions	Concentration (mM)	Relative activity (%)
Metal ions	Concentration (mM)	Lac A	Lac B
Al³⁺	1.0	58.50 ± 0.22	52.16 ± 5.71
Al³⁺	5.0	37.73 ± 0.32	34.74 ± 0.52
Mg²⁺	1.0	122.7 ± 0.70	136.8 ± 5.88
Mg²⁺	5.0	139.6 ± 2.30	157.9 ± 1.40
Zn²⁺	1.0	116.9 ± 1.72	105.3 ± 0.30
Zn²⁺	5.0	143.4 ± 2.40	136.8 ± 1.72
K⁺	1.0	115.1 ± 2.18	110.5 ± 5.48
K⁺	5.0	130.2 ± 2.06	131.6 ± 5.60
Ni²⁺	1.0	116.9 ± 0.85	147.4 ± 1.76
Ni²⁺	5.0	147.2 ± 1.21	173.4 ± 8.56
Hg²⁺	1.0	12.50 ± 0.71	35.30 ± 3.26
Hg²⁺	5.0	9.4 ± 1.14	18.40 ± 1.62
Cu²⁺	1.0	90.60 ± 1.04	82.10 ± 2.55
Cu²⁺	5.0	88.70 ± 0.99	47.40 ± 0.42
Na⁺	1.0	116.4 ± 1.77	86.80 ± 0.58
Na⁺	5.0	116.9 ± 3.01	58.40 ± 5.19
Pb²⁺	1.0	47.20 ± 1.21	50.50 ± 3.81
Pb²⁺	5.0	16.80 ± 0.30	11.60 ± 1.72

The pure laccase was incubated for 10 min in a sodium phosphate buffer (0.05 M, pH 7.0) with distinct metal ions. The reaction was then initiated with the addition of the substrate (ABTS), and the residual activity was assessed in comparison to the control with no metal ions

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ISSN: 1475-2859

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