Table 1 Purification of laccase from T. harzianum S7113
Purification step | Total Protein (mg) | Total activity (U) | Specific activity (U mg−1 protein) | Recovery (%) | Purification fold |
|---|---|---|---|---|---|
Crude | 89.36 | 97.66 | 1.09 | 100 | 1.00 |
Ammonium sulphate ppt | 22.34 | 66.66 | 2.98 | 68.25 | 2.73 |
Sephadex G-100 | 11.32 | 66.20 | 5.8 | 67.78 | 5.34 |
DEAE Sepharose | |||||
0.1 M NaCl (Lac A) | 4.46 | 41.06 | 9.19 | 42.04 | 8.41 |
0.2 M NaCl (Lac B) | 3.66 | 4.88 | 1.3 | 5.08 | 1.22 |