Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: Purification and biochemical characterization of two laccase isoenzymes isolated from Trichoderma harzianum S7113 and its application for bisphenol A degradation

Fig. 3

Effect of reaction a temperature and b pH on the activity of purified laccase isoenzymes. The buffer (0.1 M sodium citrate, pH 4.5) and substrate (ABTS) were combined and incubated at various temperatures for 5 min prior to adding the enzyme and beginning the reaction. To ascertain the effect of pH optimum on laccase activity, ABTS was utilized as a substrate in 0.1 M sodium citrate-containing buffers with pH ranges of 2.0 to 5.5

Back to article page