Table 4 Summary of the His6-tagged hanA1 fusion constructs purification results by Ni–NTA
From: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
| Â | Total protein (mg) | Yield (mg) | Purity (%) | Recovery (%) |
|---|---|---|---|---|
EmGFP | Â | Â | Â | Â |
 Ni–NTA | 22 ± 1 | 6.0 ± 0.5 | 87 ± 3 | 64 ± 2 |
 Separation/Ni–NTA | 23 ± 1 | 4.3 ± 0.3 | 91 ± 4 | 45 ± 3 |
Vhb | ||||
 Ni–NTA | 26 ± 2 | 6.7 ± 0.4 | 89 ± 3 | 66 ± 2 |
 Separation/Ni–NTA | 25 ± 2 | 5.1 ± 0.2 | 92 ± 3 | 53 ± 2 |
mSF | ||||
 Ni–NTA | 17 ± 2 | 1.2 ± 0.1 | 90 ± 3 | 61 ± 2 |
 Separation/Ni–NTA | 19 ± 2 | 1.0 ± 0.1 | 93 ± 3 | 48 ± 3 |