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Table 2 Summery of hanA1 tagged other colored proteins for affinity separation a

From: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle

 

mCherry

CrLOV

VHb

mSF

Total protein (mg) b

22 ± 2

19 ± 2

39 ± 4

18 ± 2

Purity (%)

30 ± 3

28 ± 4

15 ± 3

6 ± 3

Yield (mg)c

12 ± 1

9 ± 1

12 ± 2

2 ± 1

Purity (%)

44 ± 5

52 ± 3

39 ± 6

35 ± 3

Recovery (%)

79 ± 5

80 ± 4

77 ± 5

70 ± 2

  1. a The purification table was prepared as Table 1
  2. b Proteins were overexpressed in 200 mL LB culture
  3. c All proteins were obtained via one round of separation
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Microbial Cell Factories

ISSN: 1475-2859

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