Fig. 3
From: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
Optimization of the hanA-EmGFP separation method. A The fluorescence in soluble fractions in buffer A after CaCl2 at different concentrations were added for separation at 4 °C and 25 °C. B Fluorescence display on the SDS-PAGE gel for the correspondent protein samples under UV light. Lane 1: soluble fraction. Lane 2: the proteins left in supernatants after 10 mM CaCl2 precipitation. Lane 3: the precipitated proteins were re-solubilized with 15 mM EDTA-Na2. C SDS-PAGE analysis of the correspondent protein samples. The arrow indicated the hanA1-EmGFP