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Table 3 The levels of five independent variables employed in CCD. Coded factors are described in parentheses

From: Improved natural melanin production by Aspergillus nidulans after optimization of factors involved in the pigment biosynthesis pathway

 

Level

Factor

Symbol

-α*

Lower

Center Point

Higher

 + α*

L-tyrosine (mmol.L−1)

X1

0.02 (− 2.38)

1.4 (− 1)

2.4 (0)

3.4 (+ 1)

4.78 (+ 2.38)

Glucose (% v/v)

X2

0.11 (− 2.38)

0.8 (− 1)

1.3 (0)

1.8 (+ 1)

2.49 (+ 2.38)

Glutamic acid (mmol.L−1)

X3

0.74 (− 2.38)

3.5 (− 1)

5.5 (0)

7.5 (+ 1)

10.26 (+ 2.38)

L-DOPA (mmol.L−1)

X4

0.02 (− 2.38)

1.4 (− 1)

2.4 (0)

3.4 (+ 1)

6.85 (+ 2.38)

Copper sulfate (µmol.L−1)

X5

0.27 (− 2.38)

7.5 (− 1)

12.75 (0)

18 (+ 1)

25.23 (+ 2.38)

  1. *α = axial point