Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum

Xie, Xihui; Yang, Jianchao; Du, Hong; Chen, Jichen; Sanganyado, Edmond; Gong, Yangmin; Du, Hua; Chen, Weizhou; Liu, Zhengyi; Liu, Xiaojuan

doi:10.1186/s12934-022-02000-2

Table 1 Partially proteins with differentially N-glycosylation modification in PtFucT1 mutants

From: Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum

	Protein accession	Gene name	Site	N-gly	Description
PtFucT1 overexpression mutant
Cytoskeleton	B7G0C3	TUB-α	226	Down	Tubulin alpha chain
Cytoskeleton	B5Y3W7	TUB-β	14	Down	Tubulin beta chain
Photosynthesis	A0T0M6	psaL	112	Up	Photosystem I reaction center subunit XI
	A0T0F2	ftsH	615	Down	ATP-dependent zinc metalloprotease FtsH
	B7G503	Lhcr14	173	Down	Protein fucoxanthin chlorophyll a/c protein
	B5Y578	PetJ	103	Down	Cytochrome c-553
	A0T096	psbC	380	Down	Photosystem II CP43 reaction center protein
	A0T096	psbC	325	Down	Photosystem II CP43 reaction center protein
	B7FZ96	PsbO	68	Down	Oxygen-evolving enhancer protein 1
	Q9TK52	rbcL	313	Down	Ribulose bisphosphate carboxylase large chain
	A0T0G9	psbA	266	Down	Photosystem II protein D1
	A0T0G9	psbA	267	Down	Photosystem II protein D1
	Q9TK52	rbcL	290	Down	Ribulose bisphosphate carboxylase large chain
	B7G532	Atp1	189	Down	ATP synthase subunit alpha
	B5Y3C9	Cytb6f	74	Down	Cytochrome B6-F complex
Carbon metabolism	B7GDK9	GPI_1	472	Down	Glucose-6-phosphate isomerase
	B7G9P5	Cts	163	Down	Citrate synthase (TCA)
	B7G6K6	GapC2a	205	Down	Glyceraldehyde-3-phosphate dehydrogenase
PtFucT1 knockout mutant
Photosynthesis	B7G4U8	Lhcr6	146	Up	Protein fucoxanthin chlorophyll a/c protein
	Q9TK52	rbcL	442	Up	Ribulose bisphosphate carboxylase large chain
	Q9TK52	rbcL	37	Up	Ribulose bisphosphate carboxylase large chain
	B7FUR6	Vde	397	Up	Violaxanthin deepoxidase
Carbon metabolism	B5Y5B6	CarK	338	Up	Carbohydrate kinase
	B7G9G9	FbaC2	384	Up	Fructose-bisphosphate aldolase
	B7G518	Isol	342	Down	Isocitrate lyase
Nitrogen metabolism	B7GCN5	Agmase	284	Down	Agmatinase
Nitrogen metabolism	B7G5A1	GS	385	Down	Glutamine synthetase

Note: N-gly, N-glycosylation of the protein

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ISSN: 1475-2859

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