Fig. 8From: Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutumThe analysis of PtFucT1 and PtGnT1 interaction. 1 pBT3-N- FucT1 and pPR3-N (self-activation); 2 pBT3-N- FucT1 and pOst1-NubI (functional verification); 3 pBT3-N-FucT1 and pPR3-N- GnT1 (experimental group); + : pTSU2-APP and pNubG-Fe65 (positive control); -: pTSU2-APP and PPR3-N (negative control). SD: synthetical defined medium. DDO: SD/-Leu/-Trp nutrient deficient medium. TDO: SD/-Leu/-Trp/-His nutrient deficient mediumBack to article page