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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum

Fig. 3

Knockout mutants of PtFucT1. A chromatogram of the wild type PtFucT1 gene (1), clone expression ptCC9 with the gRNA gene (PtFucT1-836) (2) and the isolated PtFucT1 knockout mutant with 11 nucleotides deletion (3); B the sequences of 9 potential knockout mutants were certificated by online ICE; C the 9 potential knockout mutants were verified by T7 endonuclease

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