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Table 5 Experimental independent variables at two levels used for the biosynthesis of AgNPs by the supernatant of the selected bacterial isolate using Plackett–Burman design

From: Extracellular biosynthesis, OVAT/statistical optimization, and characterization of silver nanoparticles (AgNPs) using Leclercia adecarboxylata THHM and its antimicrobial activity

Variable

Symbol

level

Low (−1)

High (+ 1)

AgNO3 conc.

A

0.5 mM

1.5 mM

Bacterial supernatant conc.

B

10%

30%

incubation Time

C

24.0 h

72.0 h

pH

D

7.0

9.0

Illumination

E

Dark

Light