From: Extracellular biosynthesis, OVAT/statistical optimization, and characterization of silver nanoparticles (AgNPs) using Leclercia adecarboxylata THHM and its antimicrobial activity
Variable
Symbol
level
Low (−1)
High (+ 1)
AgNO3 conc.
A
0.5Â mM
1.5Â mM
Bacterial supernatant conc.
B
10%
30%
incubation Time
C
24.0Â h
72.0Â h
pH
D
7.0
9.0
Illumination
E
Dark
Light