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Table 1 Plackett–Burman design matrix represents the coded values of five independent variables and the absorbance readings at 420 nm as a response reflecting the AgNPs concentration

From: Extracellular biosynthesis, OVAT/statistical optimization, and characterization of silver nanoparticles (AgNPs) using Leclercia adecarboxylata THHM and its antimicrobial activity

Trials

A

B

C

D

E

Responses

Actual*

Predicted

1

 + 1

−1

−1

 + 1

−1

0.442

0.434

2

 + 1

 + 1

−1

−1

 + 1

2.199

2.081

3

 + 1

 + 1

 + 1

−1

−1

1.098

1.216

4

−1

 + 1

 + 1

 + 1

−1

0.302

0.184

5

 + 1

−1

 + 1

 + 1

 + 1

1.523

1.531

6

−1

 + 1

−1

 + 1

 + 1

0.931

1.049

7

−1

−1

 + 1

−1

 + 1

1.192

1.184

8

−1

−1

−1

−1

−1

0.079

0.087

  1. Where the five variables (A-E) are, in order, AgNO3 concentration, bacterial supernatant concentration, time, pH, and illumination, respectively. For each variable, −1 represents the low concentration level and + 1 represents the high concentration level
  2. *The experimental values were the mean absorption replicates at 420 nm