Table 2 PCR primers used in this study
Primer | Sequence (5ʹ–3ʹ) |
|---|---|
cmp12 | aaagtaagcacctgttattgc |
cmp141 | cgcagcatgtttcatttaga |
cmp150 | tgtctttgcttggcgaat |
cmp195 | gcaataacaggtgcttactttgctaccctgcaaaatatcct |
cmp196 | catatacatcctcctccatttgtatgaattcaagctttgcagg |
cmp197 | acaaatggaggaggatgtatatg |
cmp198 | aaagaaagcgatccagatgt |
cmp199 | acatctggatcgctttctttctttttcatccaatgttgctg |
pMutin2-F | gagtgtgttgatagtgcagtatc |
pMutin2-R | ctacattccctttagtaacgtgtaac |
sacA-lux-back-F | tgcagtccggcaaaaaagggc |
sacA-lux-back-R | ccaacttaatatcatcggtaggcc |
sacA-lux-check-F | ctgattggcatggcgattg |
sacA-lux-check-R | gtcgatattatgaatatcgtgccc |
sacA-lux-front-F | gacagcacatgaccaggagc |
sacA-lux-front-R | gttacacgttactaaagggaatgtagccaaaatcgtccagcccgttg |
sacA-lux-PyhaM-F | gatactgcactatcaacacactcgtatgatttatctgaaggcggtagg |
sacA-lux-PyhaM-R | gcccttttttgccggactgcacataataccagtatatcacataccgattttc |