Fig. 5
From: Development of a thiostrepton-free system for stable production of PLD in Streptomyces lividans SBT5
Optimization of carbon and nitrogen sources. A Effect of different carbon sources on PLD activity. Each concentration was 10 g/L. Columns with the same letters are not significantly different. Different lowercase letters above columns indicate statistical differences at p < 0.05. B PLD secretion efficiency and dry cell weight under different carbon sources. C The effect of various nitrogen sources on PLD activity. Each concentration was 10 g/L, and the complex nitrogen source concentration was 1:1. D Under different nitrogen sources, dry cell weight and PLD secretion efficiency were measured. E Dry cell weight and PLD secretion efficiency at various mannitol concentrations. F Optimization of the mannitol concentration in PLD activity. G Dry cell weight and PLD secretion efficiency under different ratios between ammonium sulfate and casamino acids. H Optimization of the ratio between ammonium sulfate and casamino acids in PLD activity. All data are the average of three independent experiments with standard deviations indicated