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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Bacillus integrative plasmid system combining a synthetic gene circuit for efficient genetic modifications of undomesticated Bacillus strains

Fig. 1

Scheme of plasmid transfer by conjugation. A Conjugal transfer of general plasmids from a Bacillus donor to a Bacillus recipient. The plasmid contains a replication initiator gene (rep) and its cognate double-strand origin of replication (DSO), allowing it to exit in plasmid form in both donor and recipient cells. B Conjugal transfer of integrative plasmids from a Bacillus donor to a Bacillus recipient. The integrative plasmid contains a DSO but not rep. Thus, it can be replicated in donor Bacillus cells by providing Rep in trans, but not in recipient Bacillus cells

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