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Fig. 3 | Microbial Cell Factories

Fig. 3

From: A purified and lyophilized Pseudomonas aeruginosa derived pyocyanin induces promising apoptotic and necrotic activities against MCF-7 human breast adenocarcinoma

Fig. 3

Extraction of pyocyanin pigment produced by P. aeruginosa (P 32). A Inoculation of P 32 in peptone medium supplemented with 0.3% cetrimide in a 250 ml Erlenmeyer flask; B Pyocyanin production after 3 days of incubations; C Collection of supernatant after centrifugation; D Addition of chloroform layer; E Separation of chloroform layer; F Addition of 0.2 N HCl; G Separation of the aqueous layer; H Addition of 0.2 N NaOH and restore the blue color

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Microbial Cell Factories

ISSN: 1475-2859

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