Fig. 10

Fluorescent micrographs (10 × magnification) of double-stained MCF-7 cells by propidium iodide and acridine orange (PI/AO) stains after 24 h of pyocyanin treatment. A, B Viable untreated MCF-7 cells emit green fluorescence. Red fluorescence emission indicates the formation of apoptotic and necrotic cells after 24 h treatment of MCF-7 with 0.5 IC₅₀ (C, D) and IC₅₀ (E, F) of pyocyanin. G Fluorescent micrographs (20 × magnification) of MCF-7 cells showing membrane blebbing (yellow arrow), late apoptotic cells (red arrow), and necrotic cells (green arrow). H Quantitative data representing dead and viable cell before and after pyocyanin treatment with 0.5 IC₅₀ and IC₅₀