Fig. 5

Fatty acid profiles of NLs and PLs for N. oceanica NoGPAT expression mutants. a FA compositions of NLs and PLs for exponentially growing cultures. FA contents are normalized to the total FA content per lipid class. NoGPAT-M1 and M2 showed a decrease in SFAs and an increase in PUFAs in the NLs. C18 PUFAs were further increased in PLs for these mutants, whereas C20:5 was depleted in PLs. b FA compositions for cultures after exposure to 2 days of N-depletion. NoGPAT-M1 and M2 showed an increased fraction of PUFAs and decreased fraction of SFAs, similarly to non-stressed conditions. Moreover, these mutants showed an enrichment of MUFAs in PLs, which was not seen for unstressed cultures. (a-b) Data shown are the mean \(\pm\) SD of N = 3 biological replicates (N = 5 for WT). Statistical significance was assessed by Tukey’s HSD test. (*): \(p<0.001\)