Fig. 4

Lipid contents of mutant strains during exponential growth and after exposure to N-depletion stress. a, b Non-polar lipid (NL, a) and polar lipid (PL, b) contents of mutant strains during exponential growth phase. NoGPAT and AoGPAT mutants had markedly increased NL content, whereas PL contents were similar to the wild type (NoGPAT) or slightly decreased (AoGPAT). c, d NL and PL contents after exposure to 2 days of N-depletion stress. No mutant had significantly different NL content compared to the wild type. PL contents were slightly higher for NoGPAT-M1 and M2, compared to the wild type, but substantially decreased compared to N-replete cultivation. (a-d) Relative differences compared to the WT are indicated above groups. Data shown are the mean \(\pm\) SD of N = 3 biological replicates (N = 5 for WT). Statistical significance was assessed by Tukey’s HSD test, in case of a significant ANOVA outcome. (*): \(p<0.05\); (**): \(p<0.01\); (***): \(p<0.001\)