Continuous process technology for bottom-up synthesis of soluble cello-oligosaccharides by immobilized cells co-expressing three saccharide phosphorylases

Table 2 Comparison of continuous production experiments with varying particle sizes, cell loadings, substrate concentrations, and space velocities

No.	Particle size [mm]	Cell loading [mg_CDW/g_PAM]	Sucrose/glucose [mM]	SV [h^− 1]	T [°C]	G2 yield [mM]	G3-6 yield [mM]	STY [g/L/h]	In operando [days]
1	3.00–5.00	37	300/200	0.1	45	50	0	1.7	2
2	0.25–2.00	37	300/200	0.5	45	50	7	10.0	2
3	0.25–2.00	111	300/200	0.1	30	60	91	7.6	2
4	0.25–2.00	74	300/300	0.6	30	12	27	14	1
			300/300	0.3	30	25	30	7.8	1
			300/300	0.1	30	66	49	5.2	7
5	0.25–2.00	74	300/300	0.1	30	66	71	6.6	26

Best conditions and results are marked in bold font and correspond to data shown in Fig. 6

ISSN: 1475-2859