Continuous process technology for bottom-up synthesis of soluble cello-oligosaccharides by immobilized cells co-expressing three saccharide phosphorylases

Table 1 Synthesis activities of individual enzymes provided by the free and immobilized cell catalysts before and after incubation at 45 °C for 30 h

^aSynthesis direction at 45 °C; one unit (U) of activity is the enzyme amount producing 1 µmol αGlc1-P (BaScP) or phosphate (CuCbP, CcCdP) per min under the conditions employed (see “Enzyme activity measurements” section). Standard deviations were calculated from three assay reactions with the same catalyst

ISSN: 1475-2859