Dynamic feedback regulation for efficient membrane protein production using a small RNA-based genetic circuit in Escherichia coli

Table 1 Gibbs free energies (ΔG) of the sRNA-mRNA complex and secondary structures of the unbound sRNA

	ΔG₁ (kcal/mol) sRNA-mRNA	ΔG₂ (kcal/mol) Secondary structure
Native cpxQ	− 4.29	− 17.40
cpxQmut1	− 11.36	− 17.50
cpxQmut2	− 12.04	− 16.50
cpxQmut3	− 12.12	− 18.50
cpxQmut4	− 23.46	− 14.40
cpxQmut5	− 26.94	− 14.40
cpxQmut6	− 30.20	− 14.80
cpxQmut7	− 39.52	− 6.40
cpxQmut8	− 40.07	− 6.20
cpxQmut9	− 33.90	− 14.40

ΔG₁ (kcal/mol) gives the stability of the sRNA-mRNA complex. ΔG₂ (kcal/mol) gives the ΔG of the secondary structure of the free cpxQ sRNA
mRNA messenger RNA, sRNA small RNA

ISSN: 1475-2859