Fig. 2

Summary of the disulfide bond composition of the E. coli proteome. A Number of disulfides per protein per literature source. “0.5” refers to intermolecular disulfide bonds. Not shown is one protein with 2.5 disulfide bonds identified in Chen et al. B Numbers of disulfide bond containing periplasmic proteins (top) and estimated total numbers of periplasmic disulfide bonds (calculated by multiplying the numbers of disulfide bonds in each protein with protein abundance during growth on glucose, bottom) in three data sources. Disulfide bonds identified by 2 + sources are highlighted in red. Areas in the Venn diagram are not proportional to numbers. C Number of disulfide bonds per “folding difficulty” category