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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Strategic optimization of conditions for the solubilization of GST-tagged amphipathic helix-containing ciliary proteins overexpressed as inclusion bodies in E. coli

Fig. 7

Secondary structure analysis and bioactivity of the fusion proteins using Circular Dichroism. a, b All four purified GST-fusion proteins were measured for their secondary structures using Circular Dichroism. c The alpha-helical content was quantified using the BeStSel tool (see details in the text). Note the increase in alpha helicity with the substitution of the valine with proline in the 12th position for both the amphipathic helices

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Microbial Cell Factories

ISSN: 1475-2859

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