Fig. 6

a Yields for all four fusion recombinant proteins after affinity purification using the condition of LSB-3 + BugBuster^® + SN, 3750 μL volume in the presence and absence of IGEPAL CA-630. Inset is the silver-stained SDS-PAGE gel of the purified fusion proteins. b Statistical analysis of the yield for the condition that showed maximum yield with CrFAP65AH1 purification. NS and S, respectively stand for significant and non-significant values as analyzed using one-way ANOVA and Tuckey method. Statistically significant difference between the group means F(1,4) = 8.56, p = 0.0429, F_{critical value} = 7.70 (CrFAP65AH1 ± IGEPAL CA-630); F(1,4) = 302.17, p = 0.000064, F_{critical value} = 7.70 (CrFAP65AH1V12P ± IGEPAL CA-630); F(1,4) = 28.53, p = 0.0059, F_{critical value} = 7.70 (CrFAP65AH2 ± IGEPAL CA-630) and F(1,4) = 9.60, p = 0.036, F_{critical value} = 7.70 (CrFAP65AH2V12P ± IGEPAL CA-630) wherein F statistic (df_between,df_within) = F ratio, p-value, F-critical value. The brightness and contrast of the inset gel image has been adjusted to 12%