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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Strategic optimization of conditions for the solubilization of GST-tagged amphipathic helix-containing ciliary proteins overexpressed as inclusion bodies in E. coli

Fig. 5

Yield of CrFAP65AH1 GST-fusion protein after affinity purification. a Various combination of strategies was followed for the lysis of cells as follows: BugBuster® [BB]; BugBuster® followed by Sonication [BB + SN]; LSB-2 containing 150 mM NaCl, called as LBS-3 [LSB-3]; LSB-3 with BugBuster® [LSB-3 + BB]; LSB-3 followed by Sonication [LSB-3 + SN]; LSB-3 with BugBuster® followed by Sonication [LSB-3 + BB + SN]; LSB-3 with BugBuster® and IGEPAL CA-630 [LSB-3 + BB + IG]; LSB-3 with Bugbuster® and IGEPAL followed by Sonication [LSB-3 + BB + IG + SN] were tested for their solubilization by individually purifying the fusion recombinant protein using affinity chromatography and then calculating the protein content after purification. The graph indicates the yield of the purified fusion protein in each case. b Statistical analysis of the yield for different treatments used in the purification. NSG and SG, respectively stand for significant and non-significant values as analyzed using one-way ANOVA and Tuckey method. Statistically significant difference between the group means F(7,16) = 90.83, p = 1.11E−11, Fcritical value = 2.65, wherein F statistic (dfbetween,dfwithin) = F ratio, p-value, F-critical value

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Microbial Cell Factories

ISSN: 1475-2859

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