Fig. 4

Solubilization of CrFAP65AH1-GST tagged fusion protein with LSB-3 containing 150 mM NaCl and BugBuster^® with increasing the volume of the buffer. a Induced E. coli cells after treatment with increasing buffer volume were processed for electrophoresis using 12% SDS-PAGE. b The graph indicates the band intensity of the supernatants for the induced fusion protein from the SDS-PAGE gel of a, total soluble protein in the supernatant in mg and pure protein yields post-purification using affinity chromatography. c Statistical analysis of the total soluble protein in the supernatants and yield for the increasing volume of LSB-3. NSG and SG, respectively stand for significant and non-significant values as analyzed using one-way ANOVA and Tuckey method. Statistically significant difference between the group means F(2,6) = 380.59, p = 4.78E⁻⁰⁷, F_{critical value} = 5.14, wherein F statistic (df_between,df_within) = F ratio, p-value, F-critical value. Since the band intensities were analyzed using the ImageJ tool, these are relative amounts and hence were not considered suitable for statistical analysis. The molecular weights of CrFAP65AH1 and CrFAP65AH1V12P is 29.4 kDa and that of CrFAP65AH2 and CrFAP65AH2V12P is 30.64 kDa. For the gel image, the brightness and contrast have been adjusted to 20%